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Genetic linkage map of cassava published in G3

A high-resolution linkage map and chromosome scale-genome assembly for cassava created by a group of international researchers, including some from IITA, has been published in G3 Genes Genomes Genetics and highlighted by the Genetics Society of America (http://www.g3journal.org/content/5/1/133.full).

The linkage map was generated by combining 10 genetic maps from 14 diverse parents from African cassava breeding projects including those of IITA in East Africa and Nigeria. It was a collaborative effort among IITA, the Agricultural Research Institute (ARI) of Tanzania, National Crops Resources Research Institute (NaCRRI) in Uganda, National Root Crops Research Institute  (NRCRI) in Nigeria, and the University of Berkeley/ Joint Genome Institute, USA.

It was accomplished through two collaborative projects funded by the Bill & Melinda Gates Foundation, one administered by IITA which focuses on the development of mapping populations, and the other by the University of Arizona that focuses on improving the cassava genome sequence.

According to Dr Morag Ferguson, IITA’s Cassava Molecular Geneticist, the maps have allowed the aligning of DNA sequence fragments into larger fragments or scaffolds, so that now 90% of the cassava genome assembly is contained in only 30 large fragments, whereas previously it was made up of approximately 13,000 pieces.

Cassava heap.

Cassava heap.

“This will be a valuable tool in a number of research areas from diversity assessments to functional genomics. It will ultimately assist researchers to efficiently identify and use genetic variation for improved productivity, disease resistance, and enhanced nutrition, and to develop varieties for industrial processing among other applications,” she said.

This is good news for small-holder farmers in sub-Saharan Africa where currently the crop’s production is seriously threatened by two viral diseases, cassava mosaic disease and cassava brown streak disease.

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